Overview

  • Product nameAnti-Claudin 1 antibody, prediluted
    See all Claudin 1 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 1, prediluted
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog, Monkey
  • Immunogen

    Synthetic peptide (Human) (C terminal).

  • Positive control
    • Tested with skin or breast carcinoma cells.

Properties

  • FormPrediluted
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 50mM Tris, pH 7.6
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use neat.
ICC/IF 1/200. PubMed: 17583679
IHC-P Use at an assay dependent concentration. Ready to use. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • FunctionClaudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
  • Tissue specificityStrongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
  • Involvement in diseaseIchthyosis-sclerosing cholangitis neonatal syndrome
  • Sequence similaritiesBelongs to the claudin family.
  • Cellular localizationCell junction, tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claudin-1 antibody
    • Claudin1 antibody
    • CLD 1 antibody
    • CLD1 antibody
    • CLD1_HUMAN antibody
    • CLDN 1 antibody
    • Cldn1 antibody
    • ILVASC antibody
    • SEMP 1 antibody
    • SEMP1 antibody
    • Senescence associated epithelial membrane protein 1 antibody
    • Senescence associated epithelial membrane protein antibody
    • Senescence-associated epithelial membrane protein antibody
    see all

Anti-Claudin 1 antibody, prediluted images

  • Human skin stained with ab15099.
  • Anti-Claudin 1 antibody (ab15098) at 1/25 dilution + HeLa cell lysate

    Observed band size : 19 kDa (why is the actual band size different from the predicted?)

References for Anti-Claudin 1 antibody, prediluted (ab15099)

This product has been referenced in:
  • Lim JC  et al. Neural precursor cell influences on blood-brain barrier characteristics in rat brain endothelial cells. Brain Res 1159:67-76 (2007). ICC/IF ; Rat . Read more (PubMed: 17583679) »

See 1 Publication for this product

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The concentration is 1.85 ug/ml for lot GR64050.  Please do not hesitate to contact me with any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"