Overview

  • Product nameAnti-Claudin 1 antibody [SP128]
    See all Claudin 1 primary antibodies
  • Description
    Rabbit monoclonal [SP128] to Claudin 1
  • Tested applicationsSuitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to C-terminus of Human Claudin 1.

  • Positive control
    • Human liver carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified by Protein A/G
  • ClonalityMonoclonal
  • Clone numberSP128
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionClaudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
  • Tissue specificityStrongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
  • Involvement in diseaseIchthyosis-sclerosing cholangitis neonatal syndrome
  • Sequence similaritiesBelongs to the claudin family.
  • Cellular localizationCell junction, tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claudin-1 antibody
    • Claudin1 antibody
    • CLD 1 antibody
    • CLD1 antibody
    • CLD1_HUMAN antibody
    • CLDN 1 antibody
    • Cldn1 antibody
    • ILVASC antibody
    • SEMP 1 antibody
    • SEMP1 antibody
    • Senescence associated epithelial membrane protein 1 antibody
    • Senescence associated epithelial membrane protein antibody
    • Senescence-associated epithelial membrane protein antibody
    see all

Anti-Claudin 1 antibody [SP128] images

  • Flow cytometric analysis of rabbit anti-Claudin 1 (SP128) antibody ab115783 (1/100) in HepG2 cells(green) compared to negative control of rabbit IgG (blue).

  • Staining of Human Claudin 1 in a Formalin/PFA-fixed paraffin-embedded section of Human Liver Carcinoma using ab115783 at a dilution of 1/200.

References for Anti-Claudin 1 antibody [SP128] (ab115783)

ab115783 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"