Overview

  • Product nameAnti-Claudin 2 antibody
    See all Claudin 2 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 2
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the C-terminus of Human Claudin 2.

  • Positive control
    • A431 cell lysate, Skin tissue This antibody gave a positive result when used in the following formaldehyde fixed cell line: HeLa.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab125293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).
IP Use a concentration of 2 - 5 µg/ml.
IHC-P Use a concentration of 1 - 2 µg/ml.

Target

Anti-Claudin 2 antibody images

  • ICC/IF image of ab125293 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab125293 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Claudin 2 antibody (ab125293) at 1/500 dilution + A431 cell lysate

    Predicted band size : 25 kDa

References for Anti-Claudin 2 antibody (ab125293)

This product has been referenced in:
  • Vanuytsel T  et al. From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders. PLoS One 9:e111132 (2014). WB ; Rat . Read more (PubMed: 25354336) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"