Anti-Claudin 2 antibody - Aminoterminal end (ab76032)

Overview

  • Product name
    Anti-Claudin 2 antibody - Aminoterminal end
    See all Claudin 2 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 2 - Aminoterminal end
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N terminal region of human Claudin 2

  • Positive control
    • Mouse heart tissue lysates; Human hepatocarcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notes
    ELISA: 1/1000.
    IHC-P: 1/10 - 1/50.
    WB: 1/100 - 1/250. Detects bands of approximately 25 and 50 kDa (predicted molecular weight: 25 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-Claudin 2 antibody - Aminoterminal end (ab76032) at 1/240 dilution + Mouse heart tissue lysates at 35 µg

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab76032 at a 1/10 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

    References

    This product has been referenced in:
    • Miquel S  et al. Anti-nociceptive effect of Faecalibacterium prausnitzii in non-inflammatory IBS-like models. Sci Rep 6:19399 (2016). IHC . Read more (PubMed: 26775847) »
    • Labrie V  et al. Serine racemase is associated with schizophrenia susceptibility in humans and in a mouse model. Hum Mol Genet 18:3227-43 (2009). WB ; Mouse . Read more (PubMed: 19483194) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Rhesus monkey Tissue sections (Colon)
    Specification
    Colon
    Permeabilization
    Yes - 0.1% Triton-X-100
    Fixative
    Z-fix
    Username

    Abcam user community

    Verified customer

    Submitted Oct 22 2014

    Vielen Dank für Ihren Anruf und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

    Es tut mir leid, dass Sie Probleme mit unserem Claudin 2 Antikörper haben. Da Ihre Bestellung innerhalb der letzt...

    Read More
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Pancreas)
    Loading amount
    20 µg
    Specification
    Pancreas
    Gel Running Conditions
    Reduced Denaturing (10% Bis-Tris from Invitrogen)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Joby Westmoreland

    Verified customer

    Submitted Sep 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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