Overview

  • Product name
  • Description
    Rabbit polyclonal to Claudin 23
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Pig
  • Immunogen

    Synthetic peptide derived from a region between residues 96-150 of Human Claudin 23.

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 0.5 µg/ml. Predicted molecular weight: 32 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

IHC-P Use at an assay dependent concentration.

Target

Images

  • Anti-Claudin 23 antibody (ab23355) at 1 µg/ml + HepG2 cell lysate

    Predicted band size : 32 kDa
  • All lanes : Anti-Claudin 23 antibody (ab23355) at 1 µg/ml

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 32 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa,70 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • ab23355 staining paraffin embedded human placenta tissue at 5 ug/ml.
  • ab23355 staining Claudin 23 in kidney cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with methanol, permeabilized with Triton X-100 and blocked with 5% BSA for 30 minutes. Samples were incubated with primary antibody (1/600 in PBS + 0.5% BSA + 0.1% Triton X-100) for 1 hour at 20°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/800) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Maryan N  et al. Regulation of the expression of claudin 23 by the enhancer of zeste 2 polycomb group protein in colorectal cancer. Mol Med Rep 12:728-36 (2015). Read more (PubMed: 25695204) »
  • Gareus R  et al. Normal epidermal differentiation but impaired skin-barrier formation upon keratinocyte-restricted IKK1 ablation. Nat Cell Biol 9:461-9 (2007). Read more (PubMed: 17351639) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (LUNG)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
30 µg
Specification
LUNG
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 27 2016

According to uniprot http://www.uniprot.org/uniprot/Q96B33, human Claudin 23 is a 32 kDa protein. There are not any other isoforms or cleavage fragments reported on uniprot, but Claudin 23 is not extensively studies, so there may be signal or propeptid...

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse kidney tissue)
Permeabilization
No
Specification
Mouse kidney tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Lei Pei

Verified customer

Submitted May 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney)
Specification
kidney
Fixative
Methanol
Permeabilization
Yes - Triton X
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Sep 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E14.5 wildtype epidermis)
Specification
E14.5 wildtype epidermis
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 19 2012

Merci pour votre requête.
La méthode de démasquage est une étape très importante à optimiser. Nous conseillons donc en générale d'optimiser cette étape dans chaque laboratoire.
Effectivement, si un anticorps avait fonctionné bien en HIER avec...

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Merci de nous avoir contactés.
Pour le démasquage antigène nous recommandons la méthode HIER avec du tampon Tris-EDTA pH 9.0. Comme le montre une publication de 2005 (Emoto et al., J Histochem Cytochem. 2005 Nov; 53 (11) : 1311-21, tableau nº2) le...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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