This antibody gave a positive signal in both Human Ovary and Human Colon tissue lysates as well as the following whole cell lysates: Caco2; SW480; LoVo.
This antibody gave a positive result in IHC in the following FFPE tissue: Human normal prostate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 23 kDa).
Use a concentration of 1 µg/ml.
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Involvement in diseaseNote=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the claudin family.
All lanes : Anti-Claudin 3 antibody (ab116165) at 1 µg/ml
Lane 1 : Human ovary tissue lysate - total protein (ab30222) Lane 2 : Human colon tissue lysate - total protein (ab30051) Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 5 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 6 : Human ovary tissue lysate - total protein (ab30222) with Immunizing peptide at 1 µg/ml Lane 7 : Human colon tissue lysate - total protein (ab30051) with Immunizing peptide at 1 µg/ml Lane 8 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml Lane 9 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml Lane 10 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique
IHC image of Claudin 3 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116165, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Claudin 3 antibody (ab116165)
has not yet been referenced specifically in any publications.
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