Anti-Claudin 3 (phospho Y219) antibody (ab78345)

Overview

  • Product name
    Anti-Claudin 3 (phospho Y219) antibody
    See all Claudin 3 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 3 (phospho Y219)
  • Specificity
    This antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized phosphopeptide derived from human Claudin 3 around the phosphorylation site of tyrosine 219.

  • Positive control
    • Extracts form COLO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab78345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).

Target

  • Function
    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • Involvement in disease
    Note=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities
    Belongs to the claudin family.
  • Cellular localization
    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C7orf1 antibody
    • Claudin-3 antibody
    • Claudin3 antibody
    • CLD3_HUMAN antibody
    • CLDN 3 antibody
    • Cldn3 antibody
    • Clostridium perfringens enterotoxin receptor 2 antibody
    • CPE R2 antibody
    • CPE receptor 2 antibody
    • CPE-R 2 antibody
    • CPE-receptor 2 antibody
    • CPETR 2 antibody
    • CPETR2 antibody
    • HRVP 1 antibody
    • HRVP1 antibody
    • Rat ventral prostate 1 like protein antibody
    • Rat ventral prostate.1 protein homolog antibody
    • RVP1 antibody
    • Ventral prostate.1 like protein antibody
    • Ventral prostate.1 protein homolog antibody
    see all

Images

  • All lanes : Anti-Claudin 3 (phospho Y219) antibody (ab78345) at 1/500 dilution

    Lane 1 : Extracts from COLO cells treated with EGF (200ng/ml, 30mins)
    Lane 2 : Extracts from COLO cells treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)

References

ab78345 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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