• Product nameAnti-Claudin 4 antibody
    See all Claudin 4 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 4
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide:


    , amino acids 191-209 of Human Claudin 4.

  • Positive control
    • Tested with tonsil and ovarian carcinoma cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab15104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC/IF: Use at an assay dependent dilution (PMID 19129472).
    IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
    • Involvement in diseaseNote=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • Sequence similaritiesBelongs to the claudin family.
    • Cellular localizationCell junction > tight junction. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Claudin-4 antibody
      • Claudin4 antibody
      • CLD4_HUMAN antibody
      • CLDN 4 antibody
      • CLDN4 antibody
      • Clostridium perfringens enterotoxin receptor 1 antibody
      • Clostridium perfringens enterotoxin receptor antibody
      • CPE R antibody
      • CPE receptor antibody
      • CPE-R antibody
      • CPE-receptor antibody
      • CPER antibody
      • CPETR 1 antibody
      • CPETR antibody
      • CPETR1 antibody
      • hCPE R antibody
      • WBSCR8 antibody
      • Williams Beuren syndrome chromosome region 8 protein antibody
      • Williams-Beuren syndrome chromosomal region 8 protein antibody
      see all

    Anti-Claudin 4 antibody images

    • Anti-Claudin 4 antibody (ab15104) at 1 µg/ml + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 22 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 125 kDa,34 kDa,35 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds
    • Formalin fixed paraffin embedded human tonsil stained with ab15104 (1:200, 10 min at room temperature).

    References for Anti-Claudin 4 antibody (ab15104)

    This product has been referenced in:
    • Lean QY  et al. Orally Administered Enoxaparin Ameliorates Acute Colitis by Reducing Macrophage-Associated Inflammatory Responses. PLoS One 10:e0134259 (2015). IHC ; Mouse . Read more (PubMed: 26218284) »
    • Süren D  et al. Loss of tight junction proteins (Claudin 1, 4, and 7) correlates with aggressive behavior in colorectal carcinoma. Med Sci Monit 20:1255-62 (2014). IHC-P ; Human . Read more (PubMed: 25038829) »

    See all 9 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Colon)
    Gel Running Conditions Reduced Denaturing (6-15% gradient gel)
    Loading amount 20 µg
    Specification Colon
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 28 2015

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Non-reduced Denaturing (15%)
    Sample Mouse Tissue lysate - whole (lung)
    Specification lung
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 30 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Adult Pancreas)
    Specification Adult Pancreas
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20%

    Joby Westmoreland

    Verified customer

    Submitted Mar 10 2010