Overview

  • Product name
  • Description
    Rabbit polyclonal to Claudin 4
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Extracts from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/5000.

Target

  • Function
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Involvement in disease
    Note=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities
    Belongs to the claudin family.
  • Cellular localization
    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claudin-4 antibody
    • Claudin4 antibody
    • CLD4_HUMAN antibody
    • CLDN 4 antibody
    • CLDN4 antibody
    • Clostridium perfringens enterotoxin receptor 1 antibody
    • Clostridium perfringens enterotoxin receptor antibody
    • CPE R antibody
    • CPE receptor antibody
    • CPE-R antibody
    • CPE-receptor antibody
    • CPER antibody
    • CPETR 1 antibody
    • CPETR antibody
    • CPETR1 antibody
    • hCPE R antibody
    • WBSCR8 antibody
    • Williams Beuren syndrome chromosome region 8 protein antibody
    • Williams-Beuren syndrome chromosomal region 8 protein antibody
    see all

Images

  • All lanes : Anti-Claudin 4 antibody (ab53156) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, untreated.
    Lane 2 : extracts from HeLa cells, treated with the immunizing peptide.


    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • Ab53156 staining Human colon. Staining is localised to the cell membrane.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab53156 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53156, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Pasternak JA  et al. Claudin-4 undergoes age-dependent change in cellular localization on pig jejunal villous epithelial cells, independent of bacterial colonization. Mediators Inflamm 2015:263629 (2015). IHC . Read more (PubMed: 25948883) »
  • Hwang TL  et al. Claudin-4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase-2 and -9 expression. Oncol Lett 8:1367-1371 (2014). WB ; Human . Read more (PubMed: 25120725) »

See all 4 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (intestine)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
intestine
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 6.0
Permeabilization
No
Specification
Ileum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Alex Pasternak

Verified customer

Submitted Aug 19 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chimpanzee Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Colon
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 12 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (distal ileum)
Loading amount
40 µg
Specification
distal ileum
Gel Running Conditions
Reduced Denaturing (14)
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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