Anti-Claudin 5 antibody [1G4] (ab114027)

Overview

  • Product nameAnti-Claudin 5 antibody [1G4]
    See all Claudin 5 primary antibodies
  • Description
    Mouse monoclonal [1G4] to Claudin 5
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human Claudin 5 produced in HEK293T cells (NP_003268)

  • Positive control
    • ICC/IF: Transfected COS7 cells Flow Cyt: Transfected HEK293T cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified from mouse ascites fluids by affinity chromatography
  • ClonalityMonoclonal
  • Clone number1G4
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
  • Sequence similaritiesBelongs to the claudin family.
  • Cellular localizationCell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen withdrawal and apoptosis induced protein RVP1 like antibody
    • AWAL antibody
    • BEC 1 antibody
    • BEC1 antibody
    • Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
    • Claudin-5 antibody
    • Claudin5 antibody
    • CLD5_HUMAN antibody
    • CLDN 5 antibody
    • Cldn5 antibody
    • CPETR L1 antibody
    • CPETRL 1 antibody
    • CPETRL1 antibody
    • TMDVCF antibody
    • TMVCF antibody
    • Transmembrane protein deleted in VCFS antibody
    • Transmembrane protein deleted in velocardiofacial syndrome antibody
    see all

Anti-Claudin 5 antibody [1G4] images

  • HEK293T cells transfected with either pCMV6-ENTRY CLDN5 (Red) or empty vector control plasmid (Blue) were immunostained with ab114027 at 1/100 and then analyzed by flow cytometry
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CLDN5, using ab114027 at 1/100

References for Anti-Claudin 5 antibody [1G4] (ab114027)

ab114027 has not yet been referenced specifically in any publications.

Product Wall


The lab let me know that they routinely permeabilize when doing ICC or flow cytometry (see protocol below).


This antibody was made with the full-length protein and has not been epitope-mapped thus we do not know if it detects an extr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"