Anti-Claudin 5 (phospho Y217) antibody (ab111336)

Overview

  • Product name
    Anti-Claudin 5 (phospho Y217) antibody
    See all Claudin 5 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 5 (phospho Y217)
  • Specificity
    ab111336 detects endogenous levels of Claudin 5 only when phosphorylated at tyrosine 217.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from C-terminal region of Human Claudin 5 around the phosphorylation site of Tyr217 (K-N-YP-V).

  • Positive control
    • Human brain tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111336 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Sequence similarities
    Belongs to the claudin family.
  • Cellular localization
    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen withdrawal and apoptosis induced protein RVP1 like antibody
    • AWAL antibody
    • BEC 1 antibody
    • BEC1 antibody
    • Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
    • Claudin-5 antibody
    • Claudin5 antibody
    • CLD5_HUMAN antibody
    • CLDN 5 antibody
    • Cldn5 antibody
    • CPETR L1 antibody
    • CPETRL 1 antibody
    • CPETRL1 antibody
    • TMDVCF antibody
    • TMVCF antibody
    • Transmembrane protein deleted in VCFS antibody
    • Transmembrane protein deleted in velocardiofacial syndrome antibody
    see all

Anti-Claudin 5 (phospho Y217) antibody images

  • Immunohistochemistry analysis of paraffin-embedded Human brain tissue using ab111336 at a dilution of 1/50. Left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide.

References for Anti-Claudin 5 (phospho Y217) antibody (ab111336)

ab111336 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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