Overview

  • Product name
  • Description
    Rabbit polyclonal to Claudin 6
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide between amino acids 133-163 from the central region of Human Claudin 6 (NP_067018.2), conjugated to KLH.

  • Positive control
    • Mouse heart tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab107059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 23 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

Anti-Claudin 6 antibody images

  • Anti-Claudin 6 antibody (ab107059) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 23 kDa
  • ab107059 staining Claudin 6 in murine AGDC 5 chondocytic cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldhyde and permeabilized in 0.1% Triton X-100 prior to blocking in 10% FBS in PBS for 2 hours at room temperature. The primary antibody was diluted 1/50 and incubated with the sample for 12 hours at 4°C with overnight gyratory incubation. The secondary antibody was Alexa Fluor® 594-conjugated goat anti-Rabbit polyclonal, diluted 1/50.
    Red staining is Claudin 6 and the blue is nuclear counterstaining with DAPI. Oil immersion microscopy was at 100X using an Olympus BX21 fluorescent microscope with an Infinity 3 camera.

    See Abreview

  • All lanes : Anti-Claudin 6 antibody (ab107059) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from mouse frontal cortex.
    Lane 2 : Whole tissue lysate prepared from mouse temporal cortex.
    Lane 3 : Whole tissue lysate prepared from mouse occipital cortex.
    Lane 4 : Whole tissue lysate prepared from mouse cerebellum.
    Lane 5 : Whole tissue lysate prepared from mouse brainstem.
    Lane 6 : Whole tissue lysate prepared from mouse muscle.
    Lane 7 : Whole tissue lysate prepared from mouse liver.
    Lane 8 : Whole tissue lysate prepared from mouse lung.

    Lysates/proteins at 24 µg per lane.

    Secondary
    Goat anti-rabbit IgG conjugated to HRP at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 23 kDa
    Observed band size : 23 kDa


    Exposure time : 10 seconds

    Image courtesy of Dr Gerald Davies by Abreview.

    See Abreview

  • IHC image of Claudin 6 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107059, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Claudin 6 antibody (ab107059)

ab107059 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (ovarian cancer cell line)
Permeabilization
No
Specification
ovarian cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 13 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, liver and lung)
Loading amount
24 µg
Specification
Brain, liver and lung
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Gerald Davies

Verified customer

Submitted May 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (murine AGDC 5 chondrocytic cells)
Specification
murine AGDC 5 chondrocytic cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Gerald Davies

Verified customer

Submitted May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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