For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide between amino acids 133-163 from the central region of Human Claudin 6 (NP_067018.2), conjugated to KLH.
Our Abpromise guarantee covers the use of ab107059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 23 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
Image courtesy of Dr Gerald Davies by Abreview.
IHC image of Claudin 6 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107059, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab107059 has not yet been referenced specifically in any publications.