Overview

  • Product nameAnti-Claudin 6 antibody
    See all Claudin 6 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 6
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human Claudin 6.

  • Positive control
    • human ovarian tissue, HUVEC cells, extracts from Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab75055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
  • Sequence similaritiesBelongs to the claudin family.
  • Cellular localizationCell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claudin-6 antibody
    • Claudin6 antibody
    • CLD6_HUMAN antibody
    • CLDN 6 antibody
    • CLDN6 antibody
    • OTTHUMP00000159248 antibody
    • Skullin 2 antibody
    • Skullin antibody
    • UNQ757/PRO1488 antibody
    see all

Anti-Claudin 6 antibody images

  • Human ovarian tissue with ab75055 at 1/50 dilution, in the absence and presence of the immunising peptide.
  • Huvec cells with ab75055 at 1/500 dilution, in the absence and presence of the immunising peptide.
  • All lanes : Anti-Claudin 6 antibody (ab75055) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells
    Lane 2 : extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <19 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Claudin 6 antibody (ab75055)

This product has been referenced in:
  • Florimond A  et al. Human hepatic stellate cells are not permissive for hepatitis C virus entry and replication. Gut N/A:N/A (2014). Human . Read more (PubMed: 25063678) »
  • Sarhan MA  et al. Differential expression of candidate virus receptors in human T lymphocytes prone or resistant to infection with patient-derived hepatitis C virus. PLoS One 8:e62159 (2013). WB ; Human . Read more (PubMed: 23626783) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6 25C 100C 20 min
Sample Human Tissue sections (small intestine)
Specification small intestine
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
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Abcam user community

Verified customer

Submitted Aug 07 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 22°C
Username

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Verified customer

Submitted Mar 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"