Anti-Claudin 6 antibody (ab75055)
Key features and details
- Rabbit polyclonal to Claudin 6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Claudin 6 antibody
See all Claudin 6 primary antibodies -
Description
Rabbit polyclonal to Claudin 6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from an internal sequence of human Claudin 6.
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Positive control
- human ovarian tissue, HUVEC cells, extracts from Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
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IHC-P | (2) |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9074 Human
- Omim: 615798 Human
- SwissProt: P56747 Human
- Unigene: 533779 Human
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Alternative names
- Claudin-6 antibody
- Claudin6 antibody
- CLD6_HUMAN antibody
see all
Images
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Anti-Claudin 6 antibody (ab75055) at 1/500 dilution + Jurkat cell lysate
Predicted band size: 23 kDa -
Human ovarian tissue with ab75055 at 1/50 dilution, in the absence and presence of the immunising peptide.
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Huvec cells with ab75055 at 1/500 dilution, in the absence and presence of the immunising peptide.
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All lanes : Anti-Claudin 6 antibody (ab75055) at 1/500 dilution
Lane 1 : extracts from Jurkat cells
Lane 2 : extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Additional bands at: <19 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab75055 has been referenced in 2 publications.
- Florimond A et al. Human hepatic stellate cells are not permissive for hepatitis C virus entry and replication. Gut N/A:N/A (2014). Human . PubMed: 25063678
- Sarhan MA et al. Differential expression of candidate virus receptors in human T lymphocytes prone or resistant to infection with patient-derived hepatitis C virus. PLoS One 8:e62159 (2013). WB ; Human . PubMed: 23626783