Overview

  • Product nameAnti-Claudin 6 antibody
    See all Claudin 6 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Claudin 6.

  • Positive control
    • This antibody gave a positive signal in the following lysates: A498 (Human Kidney Carcinoma) Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).

Target

  • FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
  • Sequence similaritiesBelongs to the claudin family.
  • Cellular localizationCell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claudin-6 antibody
    • Claudin6 antibody
    • CLD6_HUMAN antibody
    • CLDN 6 antibody
    • CLDN6 antibody
    • OTTHUMP00000159248 antibody
    • Skullin 2 antibody
    • Skullin antibody
    • UNQ757/PRO1488 antibody
    see all

Anti-Claudin 6 antibody images

  • Anti-Claudin 6 antibody (ab99226) at 1 µg/ml + A498 (Human Kidney Carcinoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Claudin 6 antibody (ab99226)

ab99226 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human CLDN6 overexpression)
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Loading amount 20 µg
Specification Human CLDN6 overexpression
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 02 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"