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Rabbit polyclonal to Claudin 6
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Claudin 6.
This antibody gave a positive signal in the following lysates: A498 (Human Kidney Carcinoma) Whole Cell Lysate
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
Plays a major role in tight junction-specific obliteration of the intercellular space.
Belongs to the claudin family.
Cell junction > tight junction. Cell membrane.
Information by UniProt
Western blot - Claudin 6 antibody (ab99226)
Anti-Claudin 6 antibody (ab99226) at 1 µg/ml + A498 (Human Kidney Carcinoma) Whole Cell Lysate at 10 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
24 kDa (
why is the actual band size different from the predicted?
Additional bands at:
47 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"