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Rabbit polyclonal to Claudin 6 (phospho Y219)
This antibody detects endogenous levels of Claudin 6 only when phosphorylated at tyrosine 219.
Synthesized phosphopeptide derived from human Claudin 6 around the phosphorylation site of tyrosine 219.
Extracts from RAW264.7 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Plays a major role in tight junction-specific obliteration of the intercellular space.
Belongs to the claudin family.
Cell junction > tight junction. Cell membrane.
Information by UniProt
Western blot - Claudin 6 (phospho Y219) antibody (ab78347)
All lanes : Anti-Claudin 6 (phospho Y219) antibody (ab78347) at 1/500 dilution Lane 1 : Extracts from RAW264.7 cells,
treated with UV (5mins) Lane 2 : Extracts from RAW264.7 cells,
treated with UV (5mins) with Immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 23 kDa Observed band size : 23 kDa Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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