Anti-Claudin 7/CLDN-7 antibody (ab27487)
Key features and details
- Rabbit polyclonal to Claudin 7/CLDN-7
- Suitable for: ICC, IHC-Fr, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Claudin 7/CLDN-7 antibody
See all Claudin 7/CLDN-7 primary antibodies -
Description
Rabbit polyclonal to Claudin 7/CLDN-7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Claudin 7/CLDN-7 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O95471 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Ab27487 is affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC | (1) |
Use at an assay dependent concentration.
|
IHC-Fr | (3) |
Use at an assay dependent concentration.
|
WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
|
IHC-P | (3) |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min.
|
ICC/IF |
Use at an assay dependent concentration. PubMed: 21525338
|
Notes |
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ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min. |
ICC/IF
Use at an assay dependent concentration. PubMed: 21525338 |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space. -
Tissue specificity
Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions. -
Sequence similarities
Belongs to the claudin family. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction. - Information by UniProt
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Database links
- Entrez Gene: 1366 Human
- Entrez Gene: 53624 Mouse
- Entrez Gene: 65132 Rat
- Omim: 609131 Human
- SwissProt: O95471 Human
- SwissProt: Q9Z261 Mouse
- SwissProt: Q9Z1L1 Rat
- Unigene: 513915 Human
see all -
Alternative names
- CEPTR L2 antibody
- CEPTRL 2 antibody
- CEPTRL2 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab27487 has been referenced in 39 publications.
- Xu L et al. Digital Image Analysis-Based Evaluation of Claudin-1 and Claudin-7 Delocalization in Cutaneous Squamous Cell Carcinoma and in Its Precancerous State. J Oncol 2022:2750193 (2022). PubMed: 35432533
- Wu J et al. Limosilactobacillus reuteri SLZX19-12 Protects the Colon from Infection by Enhancing Stability of the Gut Microbiota and Barrier Integrity and Reducing Inflammation. Microbiol Spectr 10:e0212421 (2022). PubMed: 35658572
- Wang K et al. Cldn-7 deficiency promotes experimental colitis and associated carcinogenesis by regulating intestinal epithelial integrity. Oncoimmunology 10:1923910 (2021). PubMed: 34026335
- Schaefer J et al. Uterine kisspeptin receptor critically regulates epithelial estrogen receptor α transcriptional activity at the time of embryo implantation in a mouse model. Mol Hum Reprod 27:N/A (2021). PubMed: 34524460
- Sfakianos JP et al. Epithelial plasticity can generate multi-lineage phenotypes in human and murine bladder cancers. Nat Commun 11:2540 (2020). PubMed: 32439865