The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min.
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 21525338
1/100. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
Tissue specificityExpressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
Ab27487, at a dilution of 1/200, staining Claudin 7 in formalin fixed paraffin embedded human colon carcinoma tissue section by Immunohistochemistry.
Immunohistochemistry (Frozen sections) - Claudin 7 antibody (ab27487)This image is courtesy of an anonymous Abreview
ab27487 staining tissue sections of rat liver after oval cell activation. The sample was PFA fixed, permeabilized in 0,1% Triton X100 for 10 minutes and blocked with 2% serum, 2%BSA, 0.05% Tween 20 in PBS for 1 hour at room temperature. The primary antibody was diluted 1/100 and incubated for 12 hours at 4°C. A HRP conjugated donkey anti-rabbit was used as the secondary.
Immunocytochemistry - Claudin 7 antibody (ab27487)This image is courtesy of an anonymous Abreview
ab27487 staining rat oval cells by ICC. Cells were fixed in ice cold methanol for 15 minutes and blocked in 3% serum for 1 hour at 25°C prior to incubation with ab27487 (diluted 1/50 in 3% Goat serum, 2% BSA, 0.01% Tween20) for 16 hours at 4°C. The secondary antibody was a Cy3® conjugated donkey anti-rabbit, diluted 1/100.
Süren D et al. Loss of tight junction proteins (Claudin 1, 4, and 7) correlates with aggressive behavior in colorectal carcinoma. Med Sci Monit20:1255-62 (2014).
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Wang H et al. Multimerization of adenovirus serotype 3 fiber knob domains is required for efficient binding of virus to desmoglein 2 and subsequent opening of epithelial junctions. J Virol85:6390-402 (2011).
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