Overview

  • Product name
  • Description
    Rabbit polyclonal to Claudin 7
  • Tested applications
    Suitable for: ICC, IHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    KAGYRAPRSYPKSNSSKEYV

    (Human) from the cytoplasmic C terminus of human Claudin 7

  • Positive control
    • Human colon carcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Ab27487 is affinity purified.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 21525338
WB 1/100. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • Function
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Tissue specificity
    Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
  • Sequence similarities
    Belongs to the claudin family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEPTR L2 antibody
    • CEPTRL 2 antibody
    • CEPTRL2 antibody
    • Claudin 1 antibody
    • Claudin 9 antibody
    • Claudin-7 antibody
    • Claudin1 antibody
    • Claudin7 antibody
    • Claudin9 antibody
    • CLD7_HUMAN antibody
    • CLDN 7 antibody
    • CLDN-7 antibody
    • CLDN7 antibody
    • Clostridium perfringens enterotoxin receptor like 2 antibody
    • CPETR L2 antibody
    • CPETRL 2 antibody
    • CPETRL2 antibody
    • Hs.84359 antibody
    see all

Anti-Claudin 7 antibody images

  • Ab27487, at a dilution of 1/200, staining Claudin 7 in formalin fixed paraffin embedded human colon carcinoma tissue section by Immunohistochemistry.
  • ab27487 staining tissue sections of rat liver after oval cell activation.  The sample was PFA fixed, permeabilized in 0,1% Triton X100 for 10 minutes and blocked with 2% serum, 2%BSA, 0.05% Tween 20 in PBS for 1 hour at room temperature.  The primary antibody was diluted 1/100 and incubated for 12 hours at 4°C.  A HRP conjugated donkey anti-rabbit was used as the secondary.

    See Abreview

  • ab27487 staining rat oval cells by ICC.  Cells were fixed in ice cold methanol for 15 minutes and blocked in 3% serum for 1 hour at 25°C prior to incubation with ab27487 (diluted 1/50 in 3% Goat serum, 2% BSA, 0.01% Tween20) for 16 hours at 4°C.  The secondary antibody was a Cy3® conjugated donkey anti-rabbit, diluted 1/100.

    See Abreview

  • All lanes : Anti-Claudin 7 antibody (ab27487) at 1/100 dilution

    Lane 1 : Whole cell lysate of human Colon Adenocarcinoma epithelial cells C2BBe1 - Insoluble fraction (membrane)
    Lane 2 : Whole cell lysate of human Colon Adenocarcinoma epithelial cells C2BBe1 - soluble fraction (cytoplasm)

    Lysates/proteins at 22 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Claudin 7 antibody (ab27487)

This product has been referenced in:
  • Tomas J  et al. High-fat diet modifies the PPAR-? pathway leading to disruption of microbial and physiological ecosystem in murine small intestine. Proc Natl Acad Sci U S A 113:E5934-E5943 (2016). IHC-P ; Mouse . Read more (PubMed: 27638207) »
  • Süren D  et al. Loss of tight junction proteins (Claudin 1, 4, and 7) correlates with aggressive behavior in colorectal carcinoma. Med Sci Monit 20:1255-62 (2014). IHC-P ; Human . Read more (PubMed: 25038829) »

See all 6 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Mature Porcine Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 6.0
Permeabilization
No
Specification
Mature Porcine Jejunum
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Alex Pasternak

Verified customer

Submitted Aug 26 2016

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (15%)
Sample
Mouse Tissue lysate - whole (lung)
Specification
lung
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako serum free protein block as blocking agent as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Monkey Tissue sections (Small intestine)
Specification
Small intestine
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 31 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Sample
Mouse Tissue sections (colon)
Specification
colon
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 04 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Non-Denaturing (Native)
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 02 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (colon)
Specification
colon
Permeabilization
Yes - boil tissue sections in 10mM citrate buffer pH 6.0
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 05 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (liver cancer)
Loading amount
20 µg
Specification
liver cancer
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Colon Adenocarcinoma epithelial cells C2BBe1)
Loading amount
22 µg
Specification
Human Colon Adenocarcinoma epithelial cells C2BBe1
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris gels)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cell (oval cells)
Specification
oval cells
Fixative
ice cold methanol 15 min
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Methanol
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 16 2008

1-10 of 12 Abreviews or Q&A

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