Overview

  • Product name
  • Description
    Rabbit polyclonal to Claudin 7
  • Specificity
    ab79481 detects endogenous levels of total Claudin 7 protein
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non phosphopeptide derived from human Claudin 7 around the phosphorylation site of tyrosine 210 (SKEYPV)

  • Positive control
    • IHC: human prostate carcinoma tissue WB: extracts from rat liver cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab79481 was purified by affinity chromatography using an epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Tissue specificity
    Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
  • Sequence similarities
    Belongs to the claudin family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEPTR L2 antibody
    • CEPTRL 2 antibody
    • CEPTRL2 antibody
    • Claudin 1 antibody
    • Claudin 9 antibody
    • Claudin-7 antibody
    • Claudin1 antibody
    • Claudin7 antibody
    • Claudin9 antibody
    • CLD7_HUMAN antibody
    • CLDN 7 antibody
    • CLDN-7 antibody
    • CLDN7 antibody
    • Clostridium perfringens enterotoxin receptor like 2 antibody
    • CPETR L2 antibody
    • CPETRL 2 antibody
    • CPETRL2 antibody
    • Hs.84359 antibody
    see all

Images

  • All lanes : Anti-Claudin 7 antibody (ab79481) at 1/500 dilution

    Lane 1 : extracts from rat liver cells
    Lane 2 : extracts from rat liver cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Claudin 7 in paraffin embedded human prostate carcinoma tissue, using ab79481 at a 1/100 dilution in the absence (left image) and presence (right image) of the immunizing peptide.

References

ab79481 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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