Anti-Claudin 7 (phospho Y210) antibody (ab78198)

Overview

  • Product name
    Anti-Claudin 7 (phospho Y210) antibody
    See all Claudin 7 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 7 (phospho Y210)
  • Specificity
    This antibody detects endogenous levels of Claudin 7 only when phosphorylated at tyrosine 210.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human Claudin 7 around the phosphorylation site of tyrosine 210 (S-K-E-YP-V).

  • Positive control
    • Extracts from rat liver cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).

Target

  • Function
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Tissue specificity
    Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
  • Sequence similarities
    Belongs to the claudin family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEPTR L2 antibody
    • CEPTRL 2 antibody
    • CEPTRL2 antibody
    • Claudin 1 antibody
    • Claudin 9 antibody
    • Claudin-7 antibody
    • Claudin1 antibody
    • Claudin7 antibody
    • Claudin9 antibody
    • CLD7_HUMAN antibody
    • CLDN 7 antibody
    • CLDN-7 antibody
    • CLDN7 antibody
    • Clostridium perfringens enterotoxin receptor like 2 antibody
    • CPETR L2 antibody
    • CPETRL 2 antibody
    • CPETRL2 antibody
    • Hs.84359 antibody
    see all

Anti-Claudin 7 (phospho Y210) antibody images

  • All lanes : Anti-Claudin 7 (phospho Y210) antibody (ab78198) at 1/500 dilution

    Lane 1 : Extracts from rat liver cells
    Lane 2 : Extracts from rat liver cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-Claudin 7 (phospho Y210) antibody (ab78198)

ab78198 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab78198.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up