The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/400.
Use 1.2µg for 106 cells. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Claudin 9 is a member of the claudin family. It is a four-transmembrane protein with WWCC motif and plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Flow cytometry analysis of BOSC23 cells transiently transfected with an expression vector encoding either Claudin 9 (red curve) or a control transfectant; using ab187116 at 1.2 µg/106 cells. PE-conjugated secondary antibodies were used for the analysis.