Overview

  • Product name
    Anti-CLC7 antibody - C-terminal
    See all CLC7 primary antibodies
  • Description
    Rabbit polyclonal to CLC7 - C-terminal
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CLC7 aa 699-728 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P51798

  • Positive control
    • CEM cell line lysate, Human kidney tissue, WiDr cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170502 is purified through a Protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 89 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the lysosome lumen.
  • Tissue specificity
    Brain, testis, muscle and kidney.
  • Involvement in disease
    Defects in CLCN7 are the cause of osteopetrosis autosomal recessive type 4 (OPTB4) [MIM:611490]; also known as infantile malignant osteopetrosis type 2. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood.
    Defects in CLCN7 are the cause of osteopetrosis autosomal dominant type 2 (OPTA2) [MIM:166600]; also known as autosomal dominant Albers-Schonberg disease or marble disease autosomal dominant. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTA2 is the most common form of osteopetrosis, occurring in adolescence or adulthood. It is characterized by sclerosis, predominantly involving the spine, the pelvis and the skull base.
  • Sequence similarities
    Belongs to the chloride channel (TC 2.A.49) family. ClC-7/CLCN7 subfamily.
    Contains 2 CBS domains.
  • Cellular localization
    Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel protein 7 antibody
    • CLC 7 antibody
    • ClC-7 antibody
    • ClC7 antibody
    • CLCN7 antibody
    • CLCN7_HUMAN antibody
    • FLJ26686 antibody
    • FLJ39644 antibody
    • FLJ46423 antibody
    • H(+)/Cl(-) exchange transporter 7 antibody
    • OPTA2 antibody
    • OPTB4 antibody
    see all

Images

  • Immunofluorescent analysis of WiDr cells labeling CLC7 with ab170502 at 1/10 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling CLC7 with ab170502 at 1/10.

  • Anti-CLC7 antibody - C-terminal (ab170502) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 89 kDa

References

ab170502 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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