Overview

  • Product nameAnti-CLCN3 antibody
    See all CLCN3 primary antibodies
  • Description
    Rabbit polyclonal to CLCN3
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: AVMTSKWVGD AFGREGIYEA HIRLNGYPFL DAKEEFTHTT LAADVMRP, corresponding to C terminal amino acids 555-604 of Human CLCN3

  • Positive control
    • Jurkat cell lysate, human brain tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab28736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 85 kDa.Can be blocked with CLCN3 peptide (ab156309). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionMediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.
  • Tissue specificityExpressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of the pulmonary vessels. Expressed in bronchus and colon (at protein level).
  • Sequence similaritiesBelongs to the chloride channel (TC 2.A.49) family. ClC-3/CLCN3 subfamily.
    Contains 2 CBS domains.
  • DomainIsoform 2 contains a C-terminal PDZ-binding motif mediating the interaction with GOPC.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationMembrane. Early endosome membrane. Late endosome membrane. Cytoplasmic vesicle > secretory vesicle membrane. Isoform 1 is localized mainly in early and late endosomes and Membrane. Early endosome membrane. Late endosome membrane. Golgi apparatus membrane. Isoform 2 partially colocalized with isoform 1 but is mainly enriched in the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel 3 antibody
    • Chloride channel protein 3 antibody
    • Chloride channel, voltage-sensitive 3 antibody
    • Chloride transporter ClC 3 antibody
    • Chloride transporter ClC-3 antibody
    • Chloride transporter ClC3 antibody
    • ClC 3 antibody
    • ClC-3 antibody
    • ClC3 antibody
    • CLCN 3 antibody
    • CLCN3 antibody
    • CLCN3_HUMAN antibody
    • DKFZp564I0463 antibody
    • H(+)/Cl(-) exchange transporter 3 antibody
    see all

Anti-CLCN3 antibody images

  • Anti-CLCN3 antibody (ab28736) at 1.25 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 85 kDa
    Observed band size : 93 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemisty analysis of human brain tissue labelling CLCN3 with ab28736.

  • ICC/IF image of ab28736 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28736, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CLCN3 antibody (ab28736) at 2.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 85 kDa

References for Anti-CLCN3 antibody (ab28736)

This product has been referenced in:
  • Mao J  et al. Lack of association between stretch-activated and volume-activated Cl? currents in hepatocellular carcinoma cells. J Cell Physiol 226:1176-85 (2011). Human . Read more (PubMed: 20945353) »

See 1 Publication for this product

Product Wall



We have added the blocking peptide for ab28736 to our catalog:
CLCN3 peptide (http://www.abcam.com/clcn3-peptide-ab156309.html)

Thank you for contacting us.

We currently have three anti-CLCN3 products on our catalog. Unfortunately these are not phospho specific antibody and will recognize CLCN3 regardless of phosphorylation state. I can suggest two courses of acti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"