Overview

  • Product nameAnti-CLCNKA antibody
    See all CLCNKA primary antibodies
  • Description
    Rabbit polyclonal to CLCNKA
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Pig
  • Immunogen

    A synthetic peptide corresponding to a region of human CLCNKA

  • Positive control
    • Fetal liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab75175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • RelevanceCLCNKA is a member of the CLC family of voltage-gated chloride channels which have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. It is predicted to have 12 transmembrane domains, and requires a beta subunit called barttin to form a functional channel. It is thought to function in salt reabsorption in the kidney, which may be important in urinary concentrating mechanisms, and potassium recycling in the inner ear.
  • Cellular localizationMembrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • C75963 antibody
    • Chloride channel Ka antibody
    • Chloride channel kidney A antibody
    • Chloride channel protein ClC Ka antibody
    • Chloride channel protein ClC-Ka antibody
    • ClC K1 antibody
    • ClC-K1 antibody
    • CLCK1 antibody
    • hClC Ka antibody
    • MGC61490 antibody
    • MGC93313 antibody
    • RP23-308N2.5 antibody
    see all

Anti-CLCNKA antibody images

  • Anti-CLCNKA antibody (ab75175) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-CLCNKA antibody (ab75175)

ab75175 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab75175.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"