Anti-Cleavage stimulation factor 2 antibody (ab64942)


  • Product nameAnti-Cleavage stimulation factor 2 antibody
    See all Cleavage stimulation factor 2 primary antibodies
  • Description
    Rabbit polyclonal to Cleavage stimulation factor 2
  • SpecificityDetects endogenous levels of total Cleavage stimulation factor 2 protein.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) from the N-terminus

  • Positive control
    • 293, HepG2 and Jurkat cell extract. HeLa cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab64942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.


  • FunctionOne of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Localized with DDX1 in cleavage bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • betaCstF 64 variant 2 antibody
    • CF-1 64 kDa subunit antibody
    • CF1 64 kDa subunit antibody
    • Cleavage stimulation factor 64 kDa subunit antibody
    • Cleavage stimulation factor subunit 2 antibody
    • Cleavage stimulation factor, 3' pre RNA, subunit 2, 64kDa antibody
    • CSTF 2 antibody
    • CstF 64 antibody
    • CSTF 64 kDa subunit antibody
    • CstF-64 antibody
    • CSTF2 antibody
    • CSTF2_HUMAN antibody
    • MGC188397 antibody
    see all

Anti-Cleavage stimulation factor 2 antibody images

  • All lanes : Anti-Cleavage stimulation factor 2 antibody (ab64942) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : Jurkat cell extract
    Lane 4 : Jurkat cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 120 kDa,20 kDa,30 kDa,48 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells, using ab64942 at 1/500 dilution. Right hand panel was also treated with immunising peptide.

References for Anti-Cleavage stimulation factor 2 antibody (ab64942)

ab64942 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Non-small cell lung cancer line (H1155))
Loading amount 25 µg
Specification Non-small cell lung cancer line (H1155)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Sep 14 2009