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Product name
Anti-cleaved IKB alpha antibody
Description
Mouse monoclonal to cleaved IKB alpha
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the caspase-3 mediated cleavage site.
Positive control
Tested with Jurkat cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Research areas
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
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Western blot - cleaved IKB alpha antibody (ab13904)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab13904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa.
Target
Relevance
NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NFkB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. Recent studies have shown that IkB alpha can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkB alpha (DNIkB alpha). The DNIkB alpha is resistant to degradation in response to inducers of NFkB and act as dominant inhibitory molecule that suppresses NFkB activity during apoptosis.
Alternative names
- IKBA antibody
- MAD3 antibody
- MAJOR HISTOCOMPATIBILITY COMPLEX ENHANCER-BINDING PROTEIN MAD3 antibody
see all
Database links
- Entrez Gene: 4792 Human
- Omim: 164008 Human
- SwissProt: P25963 Human
- Unigene: 81328 Human
Anti-cleaved IKB alpha antibody images:
Western blot - cleaved IKB alpha antibody (ab13904)
Detection of cleaved IkB alpha in Jurkat cell lysates. Lane A. untreated cell lysate. Lane B. Jurkat cells treated with anti-Fas antibody for 30 mins.
References for Anti-cleaved IKB alpha antibody (ab13904)
ab13904 has not yet been referenced specifically in any publications.
Publishing research using ab13904? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"