![Western blot - Anti-cleaved PARP antibody [194C1439] (ab13907) image](#139071.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-cleaved PARP antibody [194C1439]
See all Cleaved PARP products (10) ...
Description
Mouse monoclonal [194C1439] to cleaved PARP
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, containing amino acids 214-215 near 214/215-cleavage site of human PARP.
Positive control
camptothecin treated (12 hr) HL-60 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
194C1439
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Cell Biology >> Apoptosis >> Nucleus >> PARP
-
Western blot - Anti-cleaved PARP antibody [194C1439] (ab13907)
![Western blot - Anti-cleaved PARP antibody [194C1439] (ab13907) image](/ps/datasheet/Images/13/ab13907/139071.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1 - 2 µg/ml. Detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP09874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADP-Ribosyltransferase antibody
- ADPRT 1 antibody
- ADPRT antibody
see all
Database links
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
Anti-cleaved PARP antibody [194C1439] images:
Western blot - Anti-cleaved PARP antibody [194C1439] (ab13907)
Predicted band size : 33 kDa
Detection of cleaved PARP in HL-60 cell lysates.
Lane A. Untreated HL-60 cells.
Lane B. HL-60 cells treated with camptothecin for 12 hrs.
References for Anti-cleaved PARP antibody [194C1439] (ab13907)
This product has been referenced in:
- Greijer AE et al. Up-regulation of gene expression by hypoxia is mediated predominantly by hypoxia-inducible factor 1 (HIF-1). J Pathol 206:291-304 (2005).Read more (PubMed: 15906272) »
- Chen YC et al. Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production. Biochem Pharmacol 64:1713-24 (2002).Read more (PubMed: 12445860) »
See all 2 publications for this product
Publishing research using ab13907? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"