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Products:Cell Biology >> Apoptosis >> Nucleus >> PARP

Anti-cleaved PARP antibody [Y34] (ab32561)

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Overview

Product name

Anti-cleaved PARP antibody [Y34]
See all Cleaved PARP products (10) ...

Description

Rabbit monoclonal [Y34] to cleaved PARP

Specificity

This antibody is specific for p85 cleaved form of PARP.

Tested applications

WB, ICC, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic peptide corresponding to residues following the cleavage of site of Human PARP.

Positive control

Jurkat cell lysate.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

Y34

Isotype

IgG

Research areas

Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Cell Biology >> Apoptosis >> Nucleus >> PARP

  • Western blot - cleaved PARP antibody [Y34] (ab32561)Western blot - cleaved PARP antibody [Y34] (ab32561) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000. Predicted mo...Read more →

    WB: 1/1000. Predicted molecular weight: 85 kDa.

  • ICC

     ICC: 1/50.

    ICC: 1/50.

  • Flow Cyt

     Flow Cyt: 1/50.

    Flow Cyt: 1/50.

  • IP

     IP: 1/50.

    IP: 1/50.

Application notes

Is unsuitable for or IHC.

Target

Function

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.

Sequence similarities

Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.

Post-translational
modifications

Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP09874 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ADP-Ribosyltransferase antibody
  • ADPRT 1 antibody
  • ADPRT antibody
  • ADPRT1 antibody
  • APOPAIN antibody
  • Caspase 3 Preproprotein antibody
  • CPP32 antibody
  • CPP32B antibody
  • Cysteine Protease antibody
  • EC24230 antibody
  • MS777 antibody
  • NAD(+) ADP ribosyltransferase 1 antibody
  • NAD(+) ADP-ribosyltransferase 1 antibody
  • OTTHUMP00000035663 antibody
  • pADPRT 1 antibody
  • PARP 1 antibody
  • PARP antibody
  • PARP Cleavage Protease antibody
  • PARP-1 antibody
  • PARP1 antibody
  • PARP1_HUMAN antibody
  • Poly [ADP-ribose] polymerase 1 antibody
  • Poly ADP ribose polymerase 1 antibody
  • Poly ADP Ribose Polymerase antibody
  • Poly ADP ribose polymerase family member 1 antibody
  • Poly ADP ribose synthetase 1 antibody
  • Poly[ADP-ribose] synthase 1 antibody
  • PPOL antibody
  • SCA1 antibody
  • SREBP Cleavage Activity 1 antibody
  • YAMA antibody
see all

Database links

Anti-cleaved PARP antibody [Y34] images:

  Western blot - cleaved PARP antibody [Y34] (ab32561)

Western blot - cleaved PARP antibody [Y34] (ab32561)

All lanes : Anti-cleaved PARP antibody [Y34] (ab32561) at 1/1000 dilution

Lane 1 : Un-treated Jurkat cell lysate.
Lane 2 : Jurkat cell lysate treated with Camptothecin.


Predicted band size : 85 kDa
Observed band size : 85 kDa

References for Anti-cleaved PARP antibody [Y34] (ab32561)

This product has been referenced in:

  • Cheong JK  et al. IC261 induces cell cycle arrest and apoptosis of human cancer cells via CK1d/? and Wnt/ß-catenin independent inhibition of mitotic spindle formation. Oncogene 30:2558-69 (2011). WB; Human.Read more (PubMed: 21258417) »
  • Hornigold N  et al. Upregulation of Hic-5 in glomerulosclerosis and its regulation of mesangial cell apoptosis. Kidney Int 77:329-38 (2010). WB; Rat.Read more (PubMed: 20010548) »

See all 2 publications for this product

Publishing research using ab32561? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"