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Products:Cell Biology >> Apoptosis >> Nucleus >> PARP
Overview
Product name
Anti-Cleaved PARP antibody
See all Cleaved PARP products (10) ...
Description
Rabbit polyclonal to Cleaved PARP
Specificity
This antibody recognizes only the large fragment of PARP (89 kDa) and does not react with full length PARP. The immunogen does not contain Asp214.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
The immunogen is the N-terminus of the catalytic fragment, and does not include Asp214.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Cell Biology >> Apoptosis >> Nucleus >> PARP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 89 kDa.
ICC/IF: Use a concentration of 10 - 20 µg/ml.
Target
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP09874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADP-Ribosyltransferase antibody
- ADPRT 1 antibody
- ADPRT antibody
see all
Database links
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
References for Anti-Cleaved PARP antibody (ab2317)
This product has been referenced in:
- Lee Get al. Drosophila caspases involved in developmentally regulated programmed cell death of peptidergic neurons during early metamorphosis. J Comp Neurol 519:34-48 (2011). ICC/IF; Fruit fly (Drosophila melanogaster).Read more (PubMed: 21120926) »
- Rumpf Set al. Neuronal remodeling and apoptosis require VCP-dependent degradation of the apoptosis inhibitor DIAP1. Development 138:1153-60 (2011).Read more (PubMed: 21343367) »
See all 4 publications for this product
Publishing research using ab2317? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"