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Products:Cell Biology >> Apoptosis >> Nucleus >> PARP
Overview
Product name
Anti-Cleaved PARP antibody
See all Cleaved PARP products (10) ...
Description
Rabbit polyclonal to Cleaved PARP
Specificity
This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as marker for detecting apoptotic cells. Cleavage Site Specific Antibody, Unconjugated. The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP and will recognize Asp 214 and Gly 215.
Tested applications
ICC, WBmore details
Cross reactivity
Reacts with
Cow, Human
Immunogen
Synthetic peptide (Human) corresponding to N-terminus of cleavage site (214/215) of human PARP.
Positive control
HeLa cells treated with staurosporine at 0.5 µM for 5 hours or bovine PARP cleaved by caspase 3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a peptide spanning the cleavage site to remove antibody that is reactive with full length PARP. The final product is generated by affinity chromatography using a peptide corresponding to the PARP cleavage site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Cell Biology >> Apoptosis >> Nucleus >> PARP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/100
WB: 1/1000Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
Target
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP09874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADP-Ribosyltransferase antibody
- ADPRT 1 antibody
- ADPRT antibody
see all
Database links
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P18493 Cow
- SwissProt: P09874 Human
- Unigene: 177766 Human
Anti-Cleaved PARP antibody images:
- cleaved PARP antibody (ab4830)
Proteins from cell extracts were resolved by SDS-PAGE on a 4-20% Tris glycine gel and were transferred to PVDF membrane. Membranes were incubated with either 1
Immunocytochemistry - Cleaved PARP antibody (ab4830)
HeLa cells were induced into apoptosis with staurosporine at 0.5
Western blot - cleaved PARP antibody (ab4830)
All lanes : Anti-Cleaved PARP antibody (ab4830) at 1/1000 dilution
Lane 1 : Non-induced Jurkat cells
Lane 2 : Induced Jurkat cells
Secondary
Goat Anti-Rabbit HRP
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 85 kDa
Observed band size : 85 kDa
Exposure time : 5 seconds
This image is courtesy of an Abreview submitted by Adam Szadkowski on 26 January 2006.
References for Anti-Cleaved PARP antibody (ab4830)
This product has been referenced in:
- Przygodzka Pet al. Bomapin is a redox-sensitive nuclear serpin that affects responsiveness of myeloid progenitor cells to growth environment. BMC Cell Biol 11:30 (2010). WB; Human.Read more (PubMed: 20433722) »
- Koryllou Aet al. Cell death induced by N-methyl-N-nitrosourea, a model S(N)1 methylating agent, in two lung cancer cell lines of human origin. Apoptosis 14:1121-33 (2009). WB; Human.Read more (PubMed: 19634013) »
See all 6 publications for this product
Publishing research using ab4830? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"