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- cleaved PARP antibody (ab4830)
(enlarge)
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- cleaved PARP antibody (ab4830)
(enlarge)
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Western blot - cleaved PARP antibody (ab4830)
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Product Name
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cleaved PARP antibody
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See all PARP (cleaved) antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to cleaved PARP
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Immunogen
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Synthetic peptide (Human) corresponding to N-terminus of cleavage site (214/215) of human PARP.
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Reacts with
(species key)
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Hu, Cow
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Specificity
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This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as marker for detecting apoptotic cells. Cleavage Site
Specific Antibody, Unconjugated.
The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP and will recognize Asp 214 and Gly 215.
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Tested applications
(see key)
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ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/100.
WB: 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells treated with staurosporine at 0.5 µM for 5 hours or bovine PARP cleaved by caspase 3.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Cell Biology >> Apoptosis >> Nucleus >> PARP
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Relevance
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PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates the N-terminal DNA binding domain (24 kDa) from the C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a peptide spanning the cleavage site to remove antibody that is reactive with full length PARP. The final product is generated by affinity chromatography using a peptide corresponding to the PARP cleavage site.
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this cleaved PARP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for cleaved PARP antibody images, references, products related to ab4830 and other tools.
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cleaved PARP antibody images:
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 - cleaved PARP antibody (ab4830)
Proteins from cell extracts were resolved by SDS-PAGE on a 4-20% Tris glycine gel and were transferred to PVDF membrane. Membranes were incubated with either 1 µg/mL anti-PARP (pan) antibody or anti PARP cleavage site (214/215) specific antibody at 1 µg/mL. After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that the anti-PARP cleavage site specific antibody only recognizes the 85 kDa fragment of PARP in apoptotic cells (lane 3) and does not react with full length PARP (lane 1). The PARP (pan) antibody confirms that nonapoptotic cells express full length PARP of 116 kDa (lane 2) and which is cleaved when apoptosis is induced (lane 4).
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- cleaved PARP antibody (ab4830)
HeLa cells were induced into apoptosis with staurosporine at 0.5 µM for 5 hours (panel A) and were untreated as control (panel B). The cells were fixed in cold acetone for 5 minutes. Cells were incubated with the anti PARP cleavage site specific antibody (CCSA) at 10 µg/mL. Cells were then incubated with biotinylated goat anti-rabbit Igs followed by ABC and DAB. The data show that the anti-PARP CCSA specifically recognizes PARP in apoptotic cells. Taken together with Western blot data above, these data demonstrate the specificity of the anti-PARP CCSA for cleaved PARP.
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Western blot - cleaved PARP antibody (ab4830)
All lanes : cleaved PARP antibody (ab4830) at 1/1000 dilution
Lane 1 : Non-induced Jurkat cells
Lane 2 : Induced Jurkat cells
Secondary
Goat Anti-Rabbit HRP
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 85 kDa
Observed band size : 85 kDa
Exposure time : 5 seconds
This image is courtesy of an Abreview submitted by Adam Szadkowski on 26 January 2006.
See Abreview
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References for cleaved PARP antibody (ab4830)
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This product has been used in: (two most recent references)
Passeron T et al. Forskolin protects keratinocytes from UVB-induced apoptosis and increases DNA repair independent of its effects on melanogenesis. J Invest Dermatol 129:162-6 (2009). WB; Human. PubMed: 18580960
Ghodgaonkar MM et al. Abrogation of DNA vector-based RNAi during apoptosis in mammalian cells due to caspase-mediated cleavage and inactivation of Dicer-1. Cell Death Differ 16:858-68 (2009). WB; Human. PubMed: 19229243
See all 4 publication references for this product.
If you publish research using ab4830 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Cleaved PARP
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cleaved PARP antibody - more information
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cleaved PARP antibody - other tools:
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