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- cleaved RB antibody [ 172C1094] (ab13905)
![- cleaved RB antibody [ 172C1094] (ab13905)](/ps/datasheet/images/get_image8.jpg)
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Product Name
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cleaved RB antibody [ 172C1094]
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [ 172C1094] to cleaved RB
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Immunogen
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Synthetic peptide corresponding to region of the caspase 3 induced cleavage site of human RB was used as immunogen.
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Reacts with
(species key)
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Hu
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Specificity
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Ab13905 recognises the 68 kD fragment of cleaved RB.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysate from camptothecin induced apoptotic HL60 cells.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb
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Relevance
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Retinoblastoma gene product (RB) is a tumor suppressor protein and acts as cell cycle regulator. Recently, RB has been shown to play a role in apoptosis. During apoptosis, RB is subjected to cleavage by caspase 3 like protease. Caspase 3 cleaves RB into 48 and 68kDa fragments. Cleavage of RB would make it functionally inactive, causing release of E2F 1. The E2F 1 then may promote apoptosis.
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cleaved RB antibody [ 172C1094] (ab13905) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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172C1094
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this cleaved RB antibody [ 172C1094] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for cleaved RB antibody [ 172C1094] images, references, products related to ab13905 and other tools.
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cleaved RB antibody [ 172C1094] images:
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 - cleaved RB antibody [ 172C1094] (ab13905)
Predicted band size : 68 kDa
Western blot analysis: HL60 cells were treated for with camptothecin for 24 hours. Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. The membrane was stained with ab13905 and immunoreactivity was detected by chemiluminescense.
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Search PubMed (MEDLINE) for references to cleaved RB
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cleaved RB antibody [ 172C1094] - more information
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Customer reviews (feedback) regarding cleaved RB antibody [ 172C1094] |
Customer FAQs regarding cleaved RB antibody [ 172C1094] |
Protocols for cleaved RB antibody [ 172C1094] |
Price and availability of products related to cleaved RB antibody [ 172C1094] |
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cleaved RB antibody [ 172C1094] - related products:
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cleaved RB antibody [ 172C1094] - other tools:
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Contact Abcam with a Technical Enquiry about cleaved RB antibody [ 172C1094] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![- cleaved RB antibody [ 172C1094] (ab13905)](/index.html?datasheet=13905#get_image8.jpg)
