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Anti-cleaved RB antibody [ 172C1094] (ab13905)

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Overview

Product name

Anti-cleaved RB antibody [ 172C1094]

Description

Mouse monoclonal [ 172C1094] to cleaved RB

Specificity

Ab13905 recognises the 68 kD fragment of cleaved RB.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide corresponding to region of the caspase 3 induced cleavage site of human RB was used as immunogen.

Positive control

Whole cell lysate from camptothecin induced apoptotic HL60 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

172C1094

Isotype

IgG1

Research areas

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb

  • Western blot - Anti-cleaved RB antibody [ 172C1094] (ab13905)Western blot - Anti-cleaved RB antibody [ 172C1094] (ab13905) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 2 µg/mlDetects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

Target

Relevance

Retinoblastoma gene product (RB) is a tumor suppressor protein and acts as cell cycle regulator. Recently, RB has been shown to play a role in apoptosis. During apoptosis, RB is subjected to cleavage by caspase 3 like protease. Caspase 3 cleaves RB into 48 and 68kDa fragments. Cleavage of RB would make it functionally inactive, causing release of E2F 1. The E2F 1 then may promote apoptosis.

Cellular localization

Nuclear

Alternative names

  • Cleaved Retinoblastoma (RB) Protein antibody
  • OSRC antibody
  • Osteosarcoma antibody
  • PP110 antibody
  • RB1 antibody
  • Retinoblastoma 1 antibody
see all

Database links

Anti-cleaved RB antibody [ 172C1094] images:

  Western blot - Anti-cleaved RB antibody [ 172C1094] (ab13905)

Western blot - Anti-cleaved RB antibody [ 172C1094] (ab13905)



Predicted band size : 68 kDa


Western blot analysis: HL60 cells were treated for with camptothecin for 24 hours. Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. The membrane was stained with ab13905 and immunoreactivity was detected by chemiluminescense.

References for Anti-cleaved RB antibody [ 172C1094] (ab13905)

ab13905 has not yet been referenced specifically in any publications.

Publishing research using ab13905? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"