Recombinant
RabMAb

Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] (ab92373)

Overview

  • Product name
    Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17]
  • Description
    Rabbit monoclonal [EPR3830-17] to Cleaved alpha-fodrin (Asp1185)
  • Specificity
    Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    corresponding to Human Cleaved alpha-fodrin (Asp1185) aa 1150-1250.

  • Positive control
    • HeLa + Camptothecin cell lysate, HeLa cell lysate, Jurkat + Staurosporine cell lysate, Jurkat cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 285 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • Involvement in disease
      Epileptic encephalopathy, early infantile, 5
    • Sequence similarities
      Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 23 spectrin repeats.
    • Post-translational
      modifications
      Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
    • Cellular localization
      Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha-II spectrin antibody
      • Fodrin alpha chain antibody
      • non-erythrocytic 1 antibody
      • non-erythroid alpha subunit antibody
      • Spectrin alpha chain antibody
      • Spectrin antibody
      • SPTAN1 antibody
      • SPTN1_HUMAN antibody
      see all

    Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] images

    • All lanes : Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] (ab92373)

      Lane 1 : HeLa + Camptothecin cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat + Staurosporine cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 285 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    References for Anti-Cleaved alpha-fodrin (Asp1185) antibody [EPR3830-17] (ab92373)

    ab92373 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92373.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up