Overview

  • Product nameAnti-cleaved IKB alpha antibody
  • Description
    Mouse monoclonal to cleaved IKB alpha
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to the caspase-3 mediated cleavage site.

  • Positive control
    • Tested with Jurkat cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa.

Target

  • RelevanceNF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NFkB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. Recent studies have shown that IkB alpha can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkB alpha (DNIkB alpha). The DNIkB alpha is resistant to degradation in response to inducers of NFkB and act as dominant inhibitory molecule that suppresses NFkB activity during apoptosis.
  • Database links
  • Alternative names
    • IKBA antibody
    • MAD3 antibody
    • NFKBI antibody
    • NFKBIA antibody
    • Nuclear factor of kappa light chain gene enhancer in B-cells antibody
    see all

Anti-cleaved IKB alpha antibody images

  • Detection of cleaved IkB alpha in Jurkat cell lysates. Lane A. untreated cell lysate. Lane B. Jurkat cells treated with anti-Fas antibody for 30 mins.

References for Anti-cleaved IKB alpha antibody (ab13904)

ab13904 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"