Overview

  • Product nameAnti-Cleaved PARP antibody [194C1439]
    See all Cleaved PARP primary antibodies
  • Description
    Mouse monoclonal [194C1439] to Cleaved PARP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, containing amino acids 214-215 near 214/215-cleavage site of human PARP.

  • Positive control
    • camptothecin treated (12 hr) HL-60 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab13907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 - 2 µg/ml. Detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
    • Sequence similaritiesContains 1 BRCT domain.
      Contains 1 PARP alpha-helical domain.
      Contains 1 PARP catalytic domain.
      Contains 2 PARP-type zinc fingers.
    • Post-translational
      modifications
      Phosphorylated by PRKDC and TXK.
      Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
      S-nitrosylated, leading to inhibit transcription regulation activity.
    • Cellular localizationNucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
      • ADPRT 1 antibody
      • ADPRT antibody
      • ADPRT1 antibody
      • APOPAIN antibody
      • ARTD1 antibody
      • NAD(+) ADP-ribosyltransferase 1 antibody
      • PARP antibody
      • PARP-1 antibody
      • PARP1 antibody
      • PARP1_HUMAN antibody
      • Poly [ADP-ribose] polymerase 1 antibody
      • Poly ADP ribose polymerase 1 antibody
      • Poly[ADP-ribose] synthase 1 antibody
      • PPOL antibody
      • SCA1 antibody
      see all

    Anti-Cleaved PARP antibody [194C1439] images



    • Predicted band size : 33 kDa


      Detection of cleaved PARP in HL-60 cell lysates. Lane A. Untreated HL-60 cells. Lane B. HL-60 cells treated with camptothecin for 12 hrs.

    References for Anti-Cleaved PARP antibody [194C1439] (ab13907)

    This product has been referenced in:
    • Greijer AE  et al. Up-regulation of gene expression by hypoxia is mediated predominantly by hypoxia-inducible factor 1 (HIF-1). J Pathol 206:291-304 (2005). WB ; Mouse . Read more (PubMed: 15906272) »
    • Chen YC  et al. Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production. Biochem Pharmacol 64:1713-24 (2002). Read more (PubMed: 12445860) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your reply.

    We do have another antibody against cleaved PARP1, which has been produced in the mouse - the ab13907. http://www.abcam.com/index.html?datasheet=13907 (or use the following: http://www.abcam.com/index.html?datasheet=...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"