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Products:Cell Biology >> Apoptosis >> Nucleus >> PARP

Anti-cleaved PARP antibody (ab2321)

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Overview

Product name

Anti-cleaved PARP antibody
See all Cleaved PARP products (10) ...

Description

Rabbit polyclonal to cleaved PARP

Specificity

This antibody recognizes only the large fragment of PARP (89 kDa) and does not react with full length PARP. The immunogen contains Asp214, but not Gly215 therefore the antibody will recognise Asp214 but not Gly215.

Tested applications

IHC-Fr, WBmore details

Cross reactivity

Reacts with

Rat

Immunogen

Available on request (the immunogen contains Asp214, but not Gly215).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IHC-Fr

     IHC-Fr: Use a concentrat...Read more →

    IHC-Fr: Use a concentration of 10 - 20 µg/ml.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/mlDetects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).

Target

Function

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.

Sequence similarities

Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.

Post-translational
modifications

Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP09874 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ADP-Ribosyltransferase antibody
  • ADPRT 1 antibody
  • ADPRT antibody
  • ADPRT1 antibody
  • APOPAIN antibody
  • Caspase 3 Preproprotein antibody
  • CPP32 antibody
  • CPP32B antibody
  • Cysteine Protease antibody
  • MS777 antibody
  • NAD(+) ADP ribosyltransferase 1 antibody
  • NAD(+) ADP-ribosyltransferase 1 antibody
  • pADPRT 1 antibody
  • PARP 1 antibody
  • PARP antibody
  • PARP Cleavage Protease antibody
  • PARP-1 antibody
  • PARP1 antibody
  • PARP1_HUMAN antibody
  • Poly [ADP-ribose] polymerase 1 antibody
  • Poly ADP ribose polymerase 1 antibody
  • Poly ADP Ribose Polymerase antibody
  • Poly ADP ribose polymerase family member 1 antibody
  • Poly ADP ribose synthetase 1 antibody
  • Poly[ADP-ribose] synthase 1 antibody
  • PPOL antibody
  • SCA1 antibody
  • SREBP Cleavage Activity 1 antibody
  • YAMA antibody
see all

References for Anti-cleaved PARP antibody (ab2321)

ab2321 has not yet been referenced specifically in any publications.

Publishing research using ab2321? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"