cleaved PARP antibody (ab2321)

Product Name 

cleaved PARP antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to cleaved PARP

Immunogen 

Available on request (the immunogen contains Asp214, but not Gly215).

Reacts with 
(species key)

Rat

Specificity 

This antibody recognizes only the large fragment of PARP (89 kDa) and does not react with full length PARP. The immunogen contains Asp214, but not Gly215 therefore the antibody will recognise Asp214 but not Gly215.

Tested applications 
(see key)

IHC-Fr, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-Fr: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Cell Biology >> Apoptosis >> Nucleus >> PARP

Relevance 

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates the N-terminal DNA binding domain (24 kDa) from the C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS with 1 mg/ml BSA, 1.5 mM sodium azide and 50% glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.20 - 0.20 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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