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Products:Cell Biology >> Apoptosis >> Nucleus >> PARP
Overview
Product name
Anti-cleaved PARP antibody
See all Cleaved PARP products (10) ...
Description
Rabbit polyclonal to cleaved PARP
Specificity
This antibody recognizes only the large fragment of PARP (89 kDa) and does not react with full length PARP. The immunogen contains Asp214, but not Gly215 therefore the antibody will recognise Asp214 but not Gly215.
Tested applications
IHC-Fr, WBmore details
Cross reactivity
Reacts with
Rat
Immunogen
Available on request (the immunogen contains Asp214, but not Gly215).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Cell Biology >> Apoptosis >> Nucleus >> PARP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use a concentration of 10 - 20 µg/ml.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
Target
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP09874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADP-Ribosyltransferase antibody
- ADPRT 1 antibody
- ADPRT antibody
see all
Database links
References for Anti-cleaved PARP antibody (ab2321)
ab2321 has not yet been referenced specifically in any publications.
Publishing research using ab2321? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"