Western blot abreview for Anti-Cleaved PARP antibody [E51]

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Abreviews
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Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Treatment T3 100 micro moles for 48 hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Other product details

Incubation time 3 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: skimmed milk 5%
Dilution 1/50000

Secondary antibody

Name Non-Abcam antibody was used: goat anti rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/50000

Detection

Detection method ECL
Bands Specific: 25 kDa
Exposure 15 minute(s) and 0 second(s)

Additional data

Additional Notes the cells were treated with 100 micro moles T3 for 48 hrs and whole cell lysate was prepared by using RIPA buffer and 100 micro gram protein used for loading and 8% reduced gel used and transfered in NCM membrane and bloked by using skimmed milk and probed by using 1:50000 dil of antibody and used anti rabbit secondary antibody 1:50000 dil and used ECL method to detect and band has came at arround 25 KD
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Submitted Jun 27 2013