Anti-Cleaved PARP1 antibody [194C1439] (ab13907)

Overview

  • Product name
    Anti-Cleaved PARP1 antibody [194C1439]
    See all Cleaved PARP1 primary antibodies
  • Description
    Mouse monoclonal [194C1439] to Cleaved PARP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Cleaved PARP1. The exact sequence is proprietary. Containing amino acids 214-215 near 214/215-cleavage site of human PARP1.

  • Positive control
    • WB: Camptothecin treated (12 hr) HL-60 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 33 kDa).

Detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain.

Target

  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization
    Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Anti-Cleaved PARP1 antibody [194C1439] images



  • Predicted band size : 33 kDa

    Detection of cleaved PARP1 in HL-60 cell lysates.

    Lane A: Untreated HL-60 cells.
    Lane B: HL-60 cells treated with camptothecin for 12 hrs.

References for Anti-Cleaved PARP1 antibody [194C1439] (ab13907)

This product has been referenced in:
  • Greijer AE  et al. Up-regulation of gene expression by hypoxia is mediated predominantly by hypoxia-inducible factor 1 (HIF-1). J Pathol 206:291-304 (2005). WB ; Mouse . Read more (PubMed: 15906272) »
  • Chen YC  et al. Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production. Biochem Pharmacol 64:1713-24 (2002). Read more (PubMed: 12445860) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

We do have another antibody against cleaved PARP1, which has been produced in the mouse - the ab13907. http://www.abcam.com/index.html?datasheet=13907 (or use the following: http://www.abcam.com/index.html?datasheet=...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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