Overview

  • Product name
    Anti-Cleaved PARP1 antibody
    See all Cleaved PARP1 primary antibodies
  • Description
    Rabbit polyclonal to Cleaved PARP1
  • Specificity
    This antibody specifically recognizes the 85 kDa fragment of cleaved PARP1 and can be used as marker for detecting apoptotic cells. Cleavage site specific antibody, unconjugated. The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP1 and will recognize Asp 214 and Gly 215.
  • Tested applications
    Suitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Cleaved PARP1.
    (Peptide available as ab10779)

  • Positive control
    • HeLa cells treated with staurosporine at 0.5 µM for 5 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab4830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100.
WB 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

Target

  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization
    Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Anti-Cleaved PARP1 antibody images



  • Predicted band size : 85 kDa

    Proteins from cell extracts were resolved by SDS-PAGE on a 4-20% Tris glycine gel and were transferred to PVDF membrane. Membranes were incubated with either 1 µg/mL anti-PARP1 (pan) antibody or anti-PARP1 cleavage site (214/215) specific antibody at 1 µg/mL. After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that the anti-PARP1 cleavage site specific antibody only recognizes the 85 kDa fragment of PARP1 in apoptotic cells (lane 3) and does not react with full length PARP1 (lane 1). The PARP1 (pan) antibody confirms that nonapoptotic cells express full length PARP1 of 116 kDa (lane 2) and which is cleaved when apoptosis is induced (lane 4).

  • HeLa cells were induced into apoptosis with staurosporine at 0.5 µM for 5 hours (panel A) and were untreated as control (panel B). The cells were fixed in cold acetone for 5 minutes. Cells were incubated with the anti-PARP1 cleavage site specific antibody (CCSA) at 10 µg/mL. Cells were then incubated with biotinylated goat anti-rabbit Igs followed by ABC and DAB. The data show that the anti-PARP1 CCSA specifically recognizes PARP1 in apoptotic cells. Taken together with Western blot data above, these data demonstrate the specificity of the anti-PARP1 CCSA for cleaved PARP1.

  • All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/1000 dilution

    Lane 1 : Non-induced Jurkat cells
    Lane 2 : Induced Jurkat cells

    Secondary
    Goat Anti-Rabbit HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 5 seconds

    This image is courtesy of an Abreview submitted by Adam Szadkowski on 26 January 2006.

    See Abreview

References for Anti-Cleaved PARP1 antibody (ab4830)

This product has been referenced in:
  • Berte N  et al. Targeting Homologous Recombination by Pharmacological Inhibitors Enhances the Killing Response of Glioblastoma Cells Treated with Alkylating Drugs. Mol Cancer Ther 15:2665-2678 (2016). Read more (PubMed: 27474153) »
  • Ibrahim MY  et al. a-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-?B and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther 8:1629-47 (2014). WB ; Human . Read more (PubMed: 25302018) »

See all 11 Publications for this product

Product Wall

Application
Western blot
Sample
Human Tissue lysate - whole (Vastus lateralis muscle)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
Vastus lateralis muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (8% Gel)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted May 14 2013

Thank you for contacting Abcam with this inquiry. Unfortunately the homology between the immunogen of ab52294 and bovine Caspase-3 falls below the threshold for qualification for our Abreview testing discount. Please let me know if you have any ...

Read More

Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? Thi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (glioma cells)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Treatment
0.5 mM staurosporine in DMSO for 6hrs
Specification
glioma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 08 2010

I'm sorry to hear you are having a problem with ab4830. I did have a question about the samples you used. What kind of cells did you test? Were these treated to induce apoptosis? This antibody will only detect cleaved PARP, so if the cells are no...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Specification
Jurkat cells
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Adam Szadkowski

Verified customer

Submitted Jan 26 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up