Anti-Cleaved PARP1 antibody (ab72805)

Overview

  • Product name
    Anti-Cleaved PARP1 antibody
    See all Cleaved PARP1 primary antibodies
  • Description
    Rabbit polyclonal to Cleaved PARP1
  • Specificity
    Detects endogenous levels of fragment of activated PARP1 resulting from cleavage adjacent to Asp214.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Cleaved PARP1.

  • Positive control
    • Extracts from A549 cells, treated with etoposide (25uM, 24hours)

Properties

Applications

Our Abpromise guarantee covers the use of ab72805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
ELISA 1/20000.

Target

  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization
    Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Images

  • All lanes : Anti-Cleaved PARP1 antibody (ab72805) at 2 µg/ml

    Lane 1 : Colon cancer lysate (human)
    Lane 2 : U2OS lysate (human)
    Lane 3 : Renal Cancer Cell Line (Human)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Antibody Solutions

    Cleaved PARP1 is detected in colon cancer cell lines and osteosarcoma cells upon induction of apoptosis. In lanes 1 and 2 the cells were not treated with apoptotic agents, therefore acting as negative controls. Renal cancer cell lines express cleaved PARP1.

    See Abreview

  • All lanes : Anti-Cleaved PARP1 antibody (ab72805) at 1/1000 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with cleaved peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.Cells treated with 25 µM etoposide for 24 hours.

References

ab72805 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your call yesterday and for your patience while I have looked into your enquiry.

I have unfortunately not been able to find an antibody that I would recommend to use with drosophila samples. All of our cleaved PARP antibodies ar...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cancer (tissue lysate), U2OS (cell lysate) a)
Gel Running Conditions
Reduced Denaturing (Pierce precise protein gel 4-20%)
Loading amount
20 µg
Specification
Colon cancer (tissue lysate), U2OS (cell lysate) a
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 06 2011

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