Anti-Cleaved PARP1 antibody [E51] (HRP) (ab194217)

Overview

  • Product name
    Anti-Cleaved PARP1 antibody [E51] (HRP)
    See all Cleaved PARP1 primary antibodies
  • Description
    Rabbit monoclonal [E51] to Cleaved PARP1 (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chinese hamster
  • Immunogen

    Synthetic peptide within Human Cleaved PARP1 aa 150-250. The exact sequence is proprietary.

  • Positive control
    • WB: Jurkat whole cell treated with 10µM Camptothecin.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab194217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 98, 29 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC and TXK.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization
    Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP-ribosyltransferase diphtheria toxin-like 1 antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • APOPAIN antibody
    • ARTD1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly ADP ribose polymerase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    • SCA1 antibody
    see all

Anti-Cleaved PARP1 antibody [E51] (HRP) images

  • All lanes : Anti-Cleaved PARP1 antibody [E51] (HRP) (ab194217) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate - Treated with 10µM Camptothecin at 20 µg

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Additional bands at : 29 kDa (possible cleavage fragment),98 kDa (possible immature (unprocessed)).

    Exposure time : 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194217 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References for Anti-Cleaved PARP1 antibody [E51] (HRP) (ab194217)

ab194217 has not yet been referenced specifically in any publications.

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