Anti-cleaved RB antibody [172C1094] (ab13905)

Overview

  • Product name
    Anti-cleaved RB antibody [172C1094]
  • Description
    Mouse monoclonal [172C1094] to cleaved RB
  • Specificity
    Ab13905 recognises the 68 kD fragment of cleaved RB.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to region of the caspase 3 induced cleavage site of human RB was used as immunogen.

  • Positive control
    • Whole cell lysate from camptothecin induced apoptotic HL60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab13905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

Target

  • Relevance
    Retinoblastoma gene product (RB) is a tumor suppressor protein and acts as cell cycle regulator. Recently, RB has been shown to play a role in apoptosis. During apoptosis, RB is subjected to cleavage by caspase 3 like protease. Caspase 3 cleaves RB into 48 and 68kDa fragments. Cleavage of RB would make it functionally inactive, causing release of E2F 1. The E2F 1 then may promote apoptosis.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • OSRC antibody
    • P105 RB antibody
    • PP110 antibody
    • RB1 antibody
    • Retinoblastoma 1 antibody
    • Retinoblastoma associated protein antibody
    see all

Images



  • Predicted band size : 68 kDa


    Western blot analysis: HL60 cells were treated for with camptothecin for 24 hours. Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. The membrane was stained with ab13905 and immunoreactivity was detected by chemiluminescense.

References

ab13905 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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