Overview

  • Product name
  • Description
    Rabbit polyclonal to CLEC2A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CLEC2A.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human Spleen Tissue; Human Thymus Tissue; Human Small Intestine Tissue; Human Bone Marrow Tissue; Raji Whole Cell; Jurkat Whole Cell; MOLT4 Whole Cell.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa).

Target

  • Function
    Plays a role in modulating the extent of T-cell expansion. Enhances the expansion of TCR-stimulated T cells by increasing their survival through enhanced expression of anti-apoptotic proteins. May modulate the capacity of T-cells to home to lymph nodes through SELL. Facilitates dedicated immune recognition of keratinocytes via interaction with its receptor KLRF2 by stimulating natural killer cell mediated cytotoxicity.
  • Tissue specificity
    Mainly expressed in skin. Also expressed in keratinocytes, spleen, thymus, small intestine, peripheral blood monocytes, bone marrow, ovary, testis and skin. High expression in CD8(+), B lymphocytes and naive CD4(+) T cells. Restricted mostly to proliferating lymphocytes. Not detected in myeloid leukocytes or natural killer (NK) cells.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-type lectin domain family 2 member A antibody
    • CLC2A_HUMAN antibody
    • CLEC2A antibody
    • INPE5792 antibody
    • KACL antibody
    • Keratinocyte-associated C-type lectin antibody
    • PILAR antibody
    • Proliferation-induced lymphocyte-associated receptor antibody
    • UNQ5792 antibody
    see all

Images

  • All lanes : Anti-CLEC2A antibody (ab76711) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 7 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 300 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References

ab76711 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab76711.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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