Overview

  • Product nameAnti-CLEC2D antibody
    See all CLEC2D primary antibodies
  • Description
    Mouse monoclonal to CLEC2D
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide:

    CVTKASLPMLSPTGSPQ

    , corresponding to amino acids 2-18 of CLEC2D (OCIL).

  • Positive control
    • Mouse spleen cell lysates.
  • General notes


    Recently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin (OCIL) can also inhibit osteoclast formation in spleen cell cultures treated with RANKL and M-CSF. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its inhibitory action on adherent spleen cell cultures, which were depleted of stromal and lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase of osteoclast formation and resulted in 70% inhibition during the differentiation phase. Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol, interleukin-1 and IL-11, and retinoic acid. OCIL is expressed in osteoblasts and chondrocytes as well as in a variety of extraskeletal tissues. Since OCIL expression was found to be very similar to distribution for RANKL, it has been suggested that RANKl and OCIL may interact in skeleton and in extracellular tissues.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesRecently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin (OCIL) can also inhibit osteoclast formation in spleen cell cultures treated with RANKL and M-CSF. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its inhibitory action on adherent spleen cell cultures, which were depleted of stromal and lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase of osteoclast formation and resulted in 70% inhibition during the differentiation phase. Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol, interleukin-1 and IL-11, and retinoic acid. OCIL is expressed in osteoblasts and chondrocytes as well as in a variety of extraskeletal tissues. Since OCIL expression was found to be very similar to distribution for RANKL, it has been suggested that RANKl and OCIL may interact in skeleton and in extracellular tissues.
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 25 kDa.

Target

  • FunctionReceptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds high molecular weight sulfated glycosaminoglycans.
  • Tissue specificityDetected in peripheral blood leukocytes, osteoblasts, lymph node, thymus and spleen. Isoform 1, isoform 2 and isoform 4 are expressed in T and B lymphocytes, and at lower levels in NK cells. They are also expressed in B-cell lines and LPS-matured monocyte-derived dendritic cells.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • Cellular localizationEndoplasmic reticulum and Cell membrane. Cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin related f antibody
    • C type lectin superfamily 2, member D antibody
    • C-type lectin domain family 2 member D antibody
    • CLAX antibody
    • CLC2D_HUMAN antibody
    • CLEC2D antibody
    • CLEC2D protein antibody
    • Clec2d5 antibody
    • L LT1 antibody
    • Lectin like NK cell receptor antibody
    • Lectin like transcript 1 antibody
    • Lectin-like NK cell receptor antibody
    • Lectin-like transcript 1 antibody
    • LLT-1 antibody
    • LLT1 antibody
    • OCIL antibody
    • Osteoclast inhibitory lectin antibody
    see all

Anti-CLEC2D antibody images

  • Western blot analysis of CLEC2D (OCIL) in mouse spleen cell lysates. Thirty microgram of cell lysates per lane of mini gel was analyzed in Western blotting using ab1664.

References for Anti-CLEC2D antibody (ab13664)

This product has been referenced in:
  • Maruyama Y  et al. The effect of podoplanin inhibition on lymphangiogenesis under pathological conditions. Invest Ophthalmol Vis Sci 55:4813-22 (2014). Read more (PubMed: 24985477) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"