Overview

  • Product nameAnti-CLEC4E antibody [AT16E3]
    See all CLEC4E primary antibodies
  • Description
    Mouse monoclonal [AT16E3] to CLEC4E
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment purified from E. coli, corresponding to amino acids 41-219 of Human CLEC4E (NP_055173)

  • Positive control
    • HeLa cells and HeLa cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberAT16E3
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab100846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/250 - 1/500. Predicted molecular weight: 25 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionC-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 4 member E antibody
    • C type lectin superfamily member 9 antibody
    • C-type (calcium dependent carbohydrate recognition domain) lectin superfamily member 9 antibody
    • C-type lectin domain family 4 member E antibody
    • C-type lectin superfamily member 9 antibody
    • CLC4E_HUMAN antibody
    • CLEC 4E antibody
    • Clec4e antibody
    • CLECSF9 antibody
    • Macrophage inducible C type lectin antibody
    • Macrophage-inducible C-type lectin antibody
    • MINCLE antibody
    see all

Anti-CLEC4E antibody [AT16E3] images

  • Anti-CLEC4E antibody [AT16E3] (ab100846) at 1/1000 dilution + HeLa Cell Lysate at 35 µg

    Secondary
    goat anti-mouse conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 25 kDa
  • Immunofluorescence of Human HeLa cells stained with ab100846 (1/500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
  • ab100846 staining CLEC4E in colorectal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were subjected to antigen retrieval in 0.1M citric acid. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at room temperature. Antibody was detected by DAB staining.
  • Overlay histogram showing HeLa cells stained with ab100846 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100846, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91633, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-CLEC4E antibody [AT16E3] (ab100846)

ab100846 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"