Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation (By similarity). Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through the phosphylation of TYROBP, this interaction does not result in viral entry, but stimulates proinflammatory cytokine release.
Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon carcinoma tissue labeling CLEC5A with ab203200 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Flow Cytometry - Anti-CLEC5A antibody (ab203200)
Flow Cytometric analysis of U937 cells labeling CLEC5A using ab203200 at 1/50 dilution (green) followed by a FITC conjugated secondary, compared to control cells (blue).