Overview

  • Product name
  • Description
    Rabbit polyclonal to CLEC5A
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse CLEC5A aa 90-130 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    S ESPWKDSMDY CATQGSTLAI VNTPEKLKYL QDIAGIENYF


    Database link: Q9R007

  • Positive control
    • Human colon carcinoma tissue; U937 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab203200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20 - 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

WB 1/100 - 1/1000. Predicted molecular weight: 22 kDa.

Target

  • Function
    Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation (By similarity). Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through the phosphylation of TYROBP, this interaction does not result in viral entry, but stimulates proinflammatory cytokine release.
  • Tissue specificity
    Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    N-glycosylated. Contains sialic acid residues.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 5 member A antibody
    • C-type lectin domain family 5 member A antibody
    • C-type lectin superfamily member 5 antibody
    • CLC5A_HUMAN antibody
    • CLEC5 A antibody
    • CLEC5A antibody
    • CLECSF 5 antibody
    • CLECSF5 antibody
    • MDL 1 antibody
    • MDL-1 antibody
    • MDL1 antibody
    • Myeloid DAP12 associating lectin antibody
    • Myeloid DAP12-associating lectin 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon carcinoma tissue labeling CLEC5A with ab203200 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Flow Cytometric analysis of U937 cells labeling CLEC5A using ab203200 at 1/50 dilution (green) followed by a FITC conjugated secondary, compared to control cells (blue).

References

ab203200 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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