Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab66425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).

Target

  • FunctionFunctions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation (By similarity). Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through the phosphylation of TYROBP, this interaction does not result in viral entry, but stimulates proinflammatory cytokine release.
  • Tissue specificityExpressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • Post-translational
    modifications
    N-glycosylated. Contains sialic acid residues.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 5 member A antibody
    • C-type lectin domain family 5 member A antibody
    • C-type lectin superfamily member 5 antibody
    • CLC5A_HUMAN antibody
    • CLEC5 A antibody
    • CLEC5A antibody
    • CLECSF 5 antibody
    • CLECSF5 antibody
    • MDL 1 antibody
    • MDL-1 antibody
    • MDL1 antibody
    • Myeloid DAP12 associating lectin antibody
    • Myeloid DAP12-associating lectin 1 antibody
    see all

Anti-CLEC5A antibody images

  • Anti-CLEC5A antibody (ab66425) at 1 µg/ml + U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-CLEC5A antibody (ab66425)

ab66425 has not yet been referenced specifically in any publications.

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Canjear usando la factura original si todavía no se ha efectuado el pago. <...

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Muchas gracias por enviarnos el cuestionario relleno y las imágenes de los blots. Lamento muchísimo que el anticuerpo no os haya dado buenos resultados, y después de haber estudiado el protocolo me gustaría haceros algunas s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"