Anti-CLEC9A antibody [1F6] (ab104903)

Overview

  • Product nameAnti-CLEC9A antibody [1F6]
    See all CLEC9A primary antibodies
  • Description
    Rat monoclonal [1F6] to CLEC9A
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    RBL-2H3 cells expressing Mouse CLEC9A fused to an HA epitope.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesGrowth Conditions: RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME.
  • ClonalityMonoclonal
  • Clone number1F6
  • MyelomaY3/Ag1.2.3
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.

Target

  • FunctionFunctions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
  • Tissue specificityIn peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 9, member A antibody
    • C-type lectin domain family 9 member A antibody
    • CLC9A_HUMAN antibody
    • CLEC9A antibody
    • DNGR1 antibody
    • HEEE9341 antibody
    • PRO34046 antibody
    • UNQ9341 antibody
    see all

References for Anti-CLEC9A antibody [1F6] (ab104903)

ab104903 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: rt°C
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Submitted Aug 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"