Overview

  • Product name
    Anti-CLEC9A antibody [8F9]
    See all CLEC9A primary antibodies
  • Description
    Mouse monoclonal [8F9] to CLEC9A
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    RBL-2H3 cells expressing Human CLEC9A fused to an HA epitope

  • Positive control
    • ICC/IF: MCF7 cells.
  • General notes
    Growth Conditions: RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME.

Properties

Applications

Our Abpromise guarantee covers the use of ab104910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Block Fc receptor prior to staining to reduce background.

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
  • Tissue specificity
    In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 9, member A antibody
    • C-type lectin domain family 9 member A antibody
    • CLC9A_HUMAN antibody
    • CLEC9A antibody
    • DNGR1 antibody
    • HEEE9341 antibody
    • PRO34046 antibody
    • UNQ9341 antibody
    see all

Images

  • ICC/IF image of ab104910 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104910, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Sancho D  et al. Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest 118:2098-110 (2008). Human . Read more (PubMed: 18497879) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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