The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
FunctionFunctions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
Tissue specificityIn peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
All lanes : Anti-CLEC9A antibody (ab69364) at 1/500 dilution
Lane 1 : CLEC9A transfected 293T lysate Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 28 kDa Observed band size : 28 kDa Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
References for Anti-CLEC9A antibody (ab69364)
This product has been referenced in:
Schreibelt G et al. The C-type lectin receptor CLEC9A mediates antigen uptake and (cross-)presentation by human blood BDCA3+ myeloid dendritic cells. Blood119:2284-92 (2012).
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