Overview

  • Product nameAnti-CLIC1 antibody
    See all CLIC1 primary antibodies
  • Description
    Rabbit polyclonal to CLIC1
  • Specificityab28722 recognises Chloride intracellular channel protein 1(CLIC1)
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide derived from a region between amino acids 62-111 of human CLIC1. (Note: The exact immunogen sequence is proprietary).

  • Positive control
    • Jurkat cell lysate, Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab28722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionCan insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
  • Tissue specificityExpression is prominent in heart, placenta, liver, kidney and pancreas.
  • Sequence similaritiesBelongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • DomainMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Post-translational
    modifications
    Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
  • Cellular localizationNucleus. Nucleus membrane. Cytoplasm. Cell membrane. Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel ABP antibody
    • Chloride intracellular channel 1 antibody
    • Chloride intracellular channel protein 1 antibody
    • CLIC 1 antibody
    • CLIC1 antibody
    • CLIC1_HUMAN antibody
    • G6 antibody
    • hRNCC antibody
    • NCC 27 antibody
    • NCC27 antibody
    • Nuclear chloride ion channel 27 antibody
    • Nuclear chloride ion channel protein antibody
    • p64CLCP antibody
    • Regulatory nuclear chloride ion channel protein antibody
    • RNCC protein antibody
    see all

Anti-CLIC1 antibody images

  • Anti-CLIC1 antibody (ab28722) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 27 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of human kidney tissue labelling CLIC1 with ab28722.

References for Anti-CLIC1 antibody (ab28722)

This product has been referenced in:
  • Chen WQ  et al. Variations of protein levels in human amniotic fluid stem cells CD117/2 over passages 5-25. J Proteome Res 8:5285-95 (2009). Read more (PubMed: 19791749) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Purified protein (Human CLIC1 and CLIC4 full length protein)
Gel Running Conditions Reduced Denaturing (12% SDS gel)
Loading amount 3 µg
Specification Human CLIC1 and CLIC4 full length protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 18 2015

Application Immunoprecipitation
Immuno-precipitation step Other - Pierce Protein A/G Magnetic Beads
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Total protein in input 250 µg
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Submitted May 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (astrocytoma)
Loading amount 10 µg
Specification astrocytoma
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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Submitted Apr 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"